problems with ending particles?

The script:

repeat wait() until game.Players.LocalPlayer
local player = script.Parent.Parent
local mouse = player:GetMouse()

 mouse.KeyDown:connect(function(key)

    if key == "e" then

    player.Character.Humanoid.Sit=true






local Player = game.Players.LocalPlayer



local Particle = Instance.new("ParticleEmitter")

Particle.Parent = Player.Character.HumanoidRootPart ---
Particle.Color = Color3.new(255, 255, 255) ---

Particle.LightEmission = 1 ---

Particle.LightInfluence = 1 ---

Particle.Size = 1  ---

Particle.Texture = "rbxassetid://012345"---

Particle.Transparency = 0 ---

Particle.ZOffset = 0

Particle.EmissionDirection = "Top"

Particle.Enabled = true

Particle.Lifetime = 0, 10 ---

Particle.Rate = 5 ---

Particle.Speed = 5 ---

end
end)

how do i make the particles stop after 1 second and they dont come back until you use the script again?