Why wont my audio play when hit.Anchored == false?

You have to be more specific on line 2. You are checking if hit.Parent is a player's character. There are properties in each object such as ClassName and Name. A character's class is not Body, nor is it his/her name. That's why it's best to check if hit.Parent has a child called "Humanoid" because only players in the server have Humanoids since it gives them health.

function onTouched(hit)
    if hit.Parent:FindFirstChild("Humanoid") then
        script.Parent:Play()
    end
end

script.Parent.Touched:Connect(onTouched) -- I'm puzzled about this line because on line 3, you want the script's parent to play so it is most likely a Sound. Here, you are giving this Sound a Touched event. Sounds cannot be touched and that's why they can't have Touched events. You either made a mistake on line 3 or here. Fix this too.