The script i use isn't working? I put it in startergui

" I need everytime the player hits the text button it goes nil and after 25 worth of seconds, it becomes true again continuously "

FightingArena = game.Players.LocalPlayer.PlayerGui.FightingArena
LocalArea1 = game.Players.LocalPlayer.PlayerGui.LocalArea1
MainPoint = game.Players.LocalPlayer.PlayerGui.Mainpoint
SafeZone = game.Players.LocalPlayer.PlayerGui.SafeZone
TradeCenter1 = game.Players.LocalPlayer.PlayerGui.TradeCenter1
Overscreen = FightingArena.Overscreen
NonScreen = LocalArea1.Nonscreen
CoverScreen = MainPoint.CoverScreen
SafeZoneB = SafeZone.SafeZoneB
Cover = TradeCenter1.Cover

function False()
	FightingArena = nil
	LocalArea1 = nil
	MainPoint = nil
	SafeZone = nil
	TradeCenter1 = nil
end

Overscreen.MouseButton1Down:connect(False)
NonScreen.MouseButton1Down:connect(False)
CoverScreen.MouseButton1Down:connect(False)
SafeZoneB.MouseButton1Down:connect(False)
Cover.MouseButton1Down:connect(False)


while true do
	if False() then
		wait(25)
		FightingArena = true
		LocalArea1 = true
		MainPoint = true
		SafeZone = true
		TradeCenter1 = true
	end
end

Here is some revised code with comments on what I changed. Only LocalScripts work in starterGui so put this code in a LocalScript.

local fa = true
FightingArena = game.Players.LocalPlayer.PlayerGui.FightingArena
LocalArea1 = game.Players.LocalPlayer.PlayerGui.LocalArea1
MainPoint = game.Players.LocalPlayer.PlayerGui.Mainpoint
SafeZone = game.Players.LocalPlayer.PlayerGui.SafeZone
TradeCenter1 = game.Players.LocalPlayer.PlayerGui.TradeCenter1
Overscreen = FightingArena.Overscreen
NonScreen = LocalArea1.Nonscreen
CoverScreen = MainPoint.CoverScreen
SafeZoneB = SafeZone.SafeZoneB
Cover = TradeCenter1.Cover

function False()
    fa = false
    FightingArena.Visible = false -- If its a frame or button you need .Visible
    LocalArea1.Visible = false
    MainPoint.Visible = false
    SafeZone.Visible = false
    TradeCenter1.Visible = false
end

Overscreen.MouseButton1Down:Connect(False()) -- These need to be False() not False
NonScreen.MouseButton1Down:Connect(False())
CoverScreen.MouseButton1Down:Connect(False())
SafeZoneB.MouseButton1Down:Connect(False())
Cover.MouseButton1Down:Connect(False())


while true do
    if fa == false then
        wait(25)
        FightingArena.Visible = false
        LocalArea1.Visible = false
        MainPoint.Visible = false
        SafeZone.Visible = false
        TradeCenter1.Visible = false
    end
    wait() -- So the while loop doesn't break the game
end

Next time please provide some more elaboration on what you are trying to do.