Is there any possible way to have a GUI tween onto the screen once night falls?

Nothings impossible. To do this let's use the event LightingChanged of lighting + GetMinutesAfterMidnight() function to check

game.Lighting.LightingChanged:connect(function(skyboxchanged)
	
end)

Next let's check that it wasn't skybox that changed

game.Lighting.LightingChanged:connect(function(skyboxchanged)
	if skyboxchanged==false then

	end
end)

Also let's create a tween function + a check function with a bounce This will make it so that the tween function will only run once when it enters night

function tween()
	--stuff
end

local init = false
local last = init
function check()
	if init==true and last~=init then
		tween()
	end
	last=init
end

Now to check what time it is

game.Lighting.LightingChanged:connect(function(skyboxchanged)
	if skyboxchanged==false then
		if (game.Lighting:GetMinutesAfterMidnight()>1200 and game.Lighting:GetMinutesAfterMidnight()<1439) or (game.Lighting:GetMinutesAfterMidnight()<300 and game.Lighting:GetMinutesAfterMidnight()>0) then
			init=true
			check()
		else
			init=false
			check()
		end
	end
end)

This will check if the time in when its dark and if so it'll change init to true and fire the check function who will then check if its only once which it enters night and then fire tween

Let's put it together,

function tween()
	--stuff
end

local init = false
local last = init
function check()
	if init==true and last~=init then
		tween()
	end
	last=init
end

game.Lighting.LightingChanged:connect(function(skyboxchanged)
	if skyboxchanged==false then
		if (game.Lighting:GetMinutesAfterMidnight()>1200 and game.Lighting:GetMinutesAfterMidnight()<1439) or (game.Lighting:GetMinutesAfterMidnight()<300 and game.Lighting:GetMinutesAfterMidnight()>0) then
			init=true
			check()
		else
			init=false
			check()
		end
	end
end)

And here's it together with a tween function in the tween function (lol)

local gui = script.Parent
function tween()
	gui:Tween(stuff)
end

local init = false
local last = init
function check()
	if init==true and last~=init then
		tween()
	end
	last=init
end

game.Lighting.LightingChanged:connect(function(skyboxchanged)
	if skyboxchanged==false then
		if (game.Lighting:GetMinutesAfterMidnight()>1200 and game.Lighting:GetMinutesAfterMidnight()<1439) or (game.Lighting:GetMinutesAfterMidnight()<300 and game.Lighting:GetMinutesAfterMidnight()>0) then
			init=true
			check()
		else
			init=false
			check()
		end
	end
end)