How to make a chatted animation work with gamepass?

The problem here is: You're not referencing the player. In line three, the variable is referencing game.Players, which is a service. After fixing that, you'd have to use Player.Character in line 4 to reference the humanoid. The final problem is you didn't use WaitForChild on the animation variable. Here's a fixed version of your code:

service = game:GetService("GamePassService")
gamepass = 000000 --edit this later
player = game:GetService("Players").LocalPlayer
humanoid = player.Character:WaitForChild("Humanoid")
animation = game.Lighting:WaitForChild("Animation") 
play = humanoid:LoadAnimation(animation) 

wait() --This could possibly prevent the first player that entered from running the below code.

game:GetService("Players").PlayerAdded:Connect(function(Player)
    Player.Chatted:connect(function(Message)
           if Message == "/e test" and
              service:PlayerHasPass(player, gamepass) then
              play:Play()
        end
    end)
end)