I made a variable for the players so I can get the PlayerGui which is plrs = game.Players:GetPlayers() and I tried to check to see if a gui is visible it will remove it if there are more than 2 players and if not it will stay there. Though an error comes up saying: "ServerScriptService.Script:13: attempt to index field 'PlayerGui' (a nil value)".

plrs = game.Players:GetPlayers()

game.Players.PlayerAdded:connect(function()
    if game.Players.NumPlayers >= 2 and mapInstantiated ~= true then
		for i, v in pairs(plrs.PlayerGui:GetChildren()) do
			if v.Name == "NMP" then
				v.Visible = false
			end
		end
	else
		for i, v in pairs(plrs.PlayerGui:GetChildren()) do
			if v.Name == "NMP" then
				v.Visible = true
			end
		end
	end
	-- Rest of code

Iterate

There's something of a trick to doing something to everything, and it's that you have to iterate through your selection. In order to iterate through this, we're going to use a for loop with an iterator function - Let's have a look at how that works.

Iterator loops

Iterator loops have a simple structure:

for ... in iterator, constant, init do
	-- Code
end

But for you, we'll simplify this with an iterator factory

for k,v in pairs(table) do
	-- Code
end

What happens here is that for every time the loop body runs, k becomes the key for the table (In your case it will become the iteration count), and v becomes the value it relates to.

You already make use of an iterator loop in your code. Simply make another one to get each individual player from plrs

Your only problem is where you're attempting to get the PlayerGui of each player. This is actually a quite simple fix, you just need to understand how :GetChildren() and pairs work.

I think you pretty much have the right idea about this, but you're going about it the wrong way. The :GetChildren()method returns a table of all descendants in the object (argument in parentheses) that you include.

Pairs is used to iterate through a table of values. This means that you need to make sure that you use a table in the parentheses, which is exactly what GetChildren() returns.

Now, to apply this to your code, we simply need to move a couple things around, understanding what data types we're dealing with.

for i,v in pairs(plrs.PlayerGui:GetChildren()) do

This line of code is incorrect because plrs is a table value, and not an object (you're attempting to get the player's children, not the tables).

To fix this, we need to iterate through each object in the plrs table. Because we need to get each object before running the loop. we would now need to insert another loop:

for i,v in pairs(plrs) do
	for i2,v2 in pairs(v.PlayerGui:GetChildren()) do
		--Code
	end
end

And that's what it would look like!

Here's what your actual code would be:

plrs = game.Players:GetPlayers()

game.Players.PlayerAdded:connect(function()
	if game.Players.NumPlayers >= 2 and mapInstantiated ~= true then
		for i, v in pairs(plrs) do
			for i2,v2 in pairs(v:WaitForChild("PlayerGui"):GetChildren()) do
				if v2.Name == "NMP" then
					v2.Visible = false
				end
			end
		end
	else
		for i, v in pairs(plrs) do
			for i2,v2 in pairs(v:WaitForChild("PlayerGui"):GetChildren()) do
				if v2.Name == "NMP" then
					v.Visible = true
				end
			end
		end
	end
end
    -- Rest of code

Anyways, I hope this helped. If not, or if you have any further problems/questions, please leave a comment below, and I'll see what I can do.